procedure for physical monitoring of microbiology lab.

      1.0       Objective

     To lay down a procedure for physical monitoring of microbiology lab.

      2.0        Responsibility

      Microbiologist/QC-Executive.

      3.0        Accountability

      Head-QC executive

      4 0        Procedure

4.1       Temperature monitoring

4.1.1    Daily twice; once in morning and once in afternoon; observe the temperature of media preparation room/documentation room, incubator room, MLT room with the help of wet & dry thermo hygrometer or thermometer.

4.1.2     Record the observations in log book.

4.1.3    Microbiology laboratory environment shall be not more then 25ºC temperature. 

4.2        Pressure differential monitoring

4.2.1     Daily, once in morning, observes the pressure differential of microbiology laboratory with the help of magnehelic gauges.           

4.2.2    Record the observations in log book.

4.2.3    Microbiology laboratory environment shall be maintained at following pressure differential limits:

Sr.No	Location	Pressure Differential Limits(mm)
1.	Airlock main entry	1.2-2.0
2.	Documentation Room  /Media Preparation Room	2.8-3.6
3.	Incubator Room	2.8-3.6
4.	Airlock  (A/L to MLT room)	4.4-5.2
5.	Microbial Limit Test (MLT) Room.	6.0-6.8

4.3       In case of any abnormality observed during physical environment monitoring of microbiology department, inform to head of the department.