procedure for preparation of disinfectant and sanitization solution.

1.0       Objective      To lay down a procedure for preparation of disinfectant and sanitization solution.       2.0        Responsibility       Microbiologist/QC-Executive.       3.0       Accountability       Head-QC executive       4.0        Procedure                    Check the Manufacturing date and Expiry date before usage of disinfectant & cleaning agent.       4.1          Disinfectant / cleaning solution should  be prepared as follows:        4.1.1       Preparation of 0.2 %  Teepol solution       4.1.2    Sterilize the plastic container, for preparation of disinfectant solution by autoclaving                                                         at 121°C for 25 minutes as per SOP on Operation and Calibration of autoclave.       4.1.3     For 1 liter preparation, measure 2 ml of Teepol solution with a 10 ml measuring cylinder and transferred                       into a 1000 ml of well-cleaned and dried volumetric flask.      4.1.4      Add the purified water in the volumetric flask to make up the volume up to 1000 ml.      4.1.5      Pour this solution into a SS container. This SS container shall be transferred to LAF in   microbiology                     testing area, where it shall be filtered through sterilized filters of 0.45µm  in a sterilized filtration assembly.       4.1.6     Label the container with the details of Name of the disinfectant solution, Concentration of disinfectant                    solution, Prepared on, Use before and Prepared by.       4.2        Preparation of 2.5% Dettol solution       4.2.1    Sterilize the plastic container, for preparation of disinfectant solution by autoclaving at 121°C for 25                    minutes   as per SOP on Operation and Calibration of autoclave.       4.2.2    For 1 liter preparation, measure 25 ml of Dettol solution with a 25 ml measuring  cylinder and transferred                    into a 1000 ml of wel-cleaned and dried volumetric flask       4.2.3     Add the purified water in the volumetric flask to make up the volume up to 1000 ml.                     4.2.4     Pour this solution into a SS container. This SS container shall be transferred to LAF in microbiology testing                 area, where it shall be filtered through sterilized filters of 0.45µ in a sterilized filtration assembly.   4.2.5     Label the container with the details of Name of the disinfectant solution Concentration of disinfectant             solution, Prepared on, Use before and Prepared by.       4.3   Preparation of 2.5%   Savlon  solution     4.3.1      Sterilize the plastic container, for preparation of disinfectant solution by autoclaving at 121°C for 25                    minutes as per SOP on Operation and Calibration of autoclave.      4.3.2      For, 1 liter preparation, measure 25 ml of Savlon solution with a 25 ml measuring cylinder and transferred                     into a 1000 ml of well-cleaned and dried volumetric flask.      4.3.3      Add the purified water in the volumetric flask to make up the volume up to 1000 ml.      4.3.4       Pour this solution into a SS container. This SS container shall be transferred to LAF in microbiology                     testing area, where it shall be filtered through sterilized filters of 0.45µ in a sterilized filtration assembly.        4.4        Preparation of 70% Iso Propyl Alcohol solution      4.4.1      For 1liter preparation, measured 700 ml of IPA solution with a 1000 ml measuring cylinder and transferred                     into a 1000 ml of well-cleaned and dried volumetric flask.       4.4.2     Add the purified water in the volumetric flask to make up the volume up to 1000 ml.       4.4.3     Pour this solution into a SS container. This SS container shall be transferred in LAF in   microbiology                               testing area, where it shall be filtered through sterilized filter of 0.45µm in a sterilized filtration assembly. 4.4.4   Label the container with the details of Name of the disinfectant solution, Concentration of  disinfectant              solution, Prepared on, Use before and Prepared by        5.0       Reference:                       In house          6.0       Format DQM/03/F/01-01         : Preparation of disinfectant  sol. record       7.0     Abbreviations(S) QC              : QUALITY CONTROL DQM DAGON QC MICRO LAB       8.0        Revision LOG : Revision No. : Reason for change 00 : Initial 01 : Format Change