procedure for cleaning and sterlization of glassware and garments

1.0       Objective      To lay down a procedure for cleaning and sterlization of glassware and garments.       2.0        Responsibility       Microbiologist/QC-Executive.       3.0        Accountability       Head-QC executive       4.0         Procedure       4.1        Cleaning of Glassware                                                    4.1.1       The cleaning of glassware should be done as follows:       4.1.2       All the media in the used Petri plates and test tubes should be removed and collected in disposal bag.       4.1.3       The glassware should be transferred in washing area.       4.1.4       The glassware washed with potable water, followed by washing with teepol solution and again with soft                         water.       4.1.5       Finally the glassware washed with purified water to remove the traces of Teepol solution in / on the                       glassware. 4.1.6      The glassware should be dried in the Hot air oven.        4.2             Sterilization of glassware       4.2.1        Sterilized Glassware in autoclave.       4.2.2        All the Petri plates shall be individually wrapped in a single layer of butter paper or directly place in                        S.S. Container.       4.2.3        Place these wrapped Petri plates in a S.S container and sterilized by autoclaving at 121°C Screw cap                      sampling bottles shall be loosely capped and sterilized by autoclaving at 121°C for 15 minutes in                      autoclave.        4.2.4      After the completion of the sterilization cycle, autoclave is opened; these bottles shall be screwed                          tightly.        4.2.5      Test tubes are plugged with cotton when sterilized in autoclave.        4.3         Sterilization of Garments          .        4.3.1      Used garments shall be sent to laundry for washing.       4.3.2       After receiving washed garments from laundry, the same shall be put in Hi-media bag for                                                sterilization.                4.3.3        Place these bags (wrapped garments) in autoclave for sterilization at 121°C for 25 minutes.      4.3.4        Subsequently the dried clothes bag shall be kept in cabinet of the change room for use.