procedure for preparation of settle plate and slants.

      1.0       Objective

     To lay down a procedure for preparation of settle plate and slants.

      2.0        Responsibility

      Microbiologist/QC-Executive.

      3.0        Accountability

      Head-QC executive

      4.0        Procedure

      4.1         Preparation of settle plates

4.1.1          Prepare the media as per SOP.

4.1.2          After sterilization, cool down the media to approximately 40°C -45 °C.

4.1.3          Aseptically pour 15-20 ml media in sterile petriplates base, then cover the glass lid and allow it to solidify under Laminar Air Flow (LAF).

4.1.4          Label the base of petriplates.

4.1.5          Label shall include:

            1.       Name of the media:

            2.       Date of preparation:

            3.       Signature:

4.1.6          Preincubate the Soyabean Casein Digest Agar (SCDA) medium plates or any other media plates for enumeration of bacteria, at 30° to 35° C for 48 hours in inverted position.

4.1.7          Preincubate the Sabouraud dextrose agar (SDA) medium plates or any other media plates for enumeration of fungi, at 20° to 25° C for 72 hours in inverted position.

4.1.8          After incubation observe the plate whether it is contaminated or not.

4.1.9          Discard the contaminated plates.

4.1.10      These preincubated (non-contaminated) plates are ready for use.

4.2                Preparation of slants

4.3                Prepare the media as per SOP.

4.2.1         Dispense 10 mL media in glass test tube and plug the slants with nonabsorbent cotton.

4.2.2          After sterilization of media keep the test tubes in slanting position and allow it to solidify.

4.2.3         Label the top of slant tubes.

4.2.4         Label shall include:

           1.       Name of the media:

           2.       Date of preparation:

           3.       Signature:

4.2.5        Preincubate the Soyabean Casein Digest Agar (SCDA) medium slants or any other media slants for enumeration of bacteria, at 30° to 35° C for 48 hours.

4.2.6        Preincubate the Sabouraud dextrose Agar (SDA) medium slants or any other media slants for enumeration of fungi, at 20° to 25° C for 72 hours.

4.2.7        After incubation, observe the slant whether it is contaminated or not.

4.2.8        Discard the contaminated slants.

4.2.9        These preincubated (non-contaminated) slants are ready for use